ChoiceTaqTM Blue Mastermix is a complete “ready-to-go” 2X reaction mix which only requires the users to add water, template and primers. The product is supplied with Blue Taq DNA polymerase, dNTP mix, and PCR reaction buffer containing MgCl2, which produces afinalMg2+ concentration of 1.5mM. Blue Taq differs from regular Taq in that it contains blue dye for direct gel loading after PCR reaction with no need of any loading buffer. It is ideal forprimary extension reaction with DNA fragments having dA overhang on 3’ ends; at extension speed of 1200 bases per minute and optimized between 65-75°C.