3G HotStart Taq DNA Polymerase (5u/ul): HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures.
During the initial denaturation step at 94°C, the antibody dissociates from DNA polymerase and restores enzyme activity.
This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability, thus offering higher specificity and improved yield of PCR products.
PCR products amplified up to 6 kb in length with HotStart DNA Polymerase contain a single base (A) 3’ overhang.