NuSieveTM 3:1 Agarose is a molecular biology grade, standard melting temperature agarose that yields strong gels for fine resolution of small DNA, RNA, and PCR products ≤ 1 kb. NuSieveTM 3:1 Agarose is designed for analytical electrophoresis rather than in gel reactions or downstream applications. These easy-to-handle gels enhance the speed of gel processing and blotting. Performance testing of NuSieveTM 3:1 Agarose ensures fine resolution of DNA fragments up to 1,000 bp, though this agarose is capable of finely resolving DNA fragments ranging from 10 bp up to 1,500 bp.
Benefits:
- All Lonza Agarose is quality tested to certify performance
- High gel strength
- Exceptional resolution of 50 bp to 1000 bp DNA fragments
- Standard melting temperature
Applications:
- Fine/Small Fragment Electrophoreisis
Analytical Specifications:
Gelling temperature (4%) 32.5 - 38.0°C
Melting temperature (4%) ≤90°C
Gel strength (4%) ≥1,400 g/cm²
PCR Products on a NuSieve™ 3:1 Agarose Gel – A 550 bp sequence from lambda DNA was amplified (25 cycles) using primers and Taq DNA polymerase supplied in the GeneAmp® kit (Roche Molecular Systems). PCR products and controls were electro phoresed on a 4% NuSieve™ 3:1 agarose gel in TAE buffer at 5 V/cm for 3 hours. Lane 1, Msp I digest of pBR322 DNA (1.5 μg); lane 2, Hae III digest of øX174 DNA (1.5 μg); lane 3, no DNA control; lanes 4 – 9, PCR products resulting from different reaction conditions (7 μl of 100 μl reaction mixture); and lane 10, a positive control where kit template was added.