A selection of genomic isolation kits are offered that purify high quality genomic DNA from a wide variety of sources and for a wide array of applications.
Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications. These kits are fully scalable for large genomic DNA isolations. The procedure is slightly more involved to ensure ultra pure DNA.
Based on our popular OmniPrep™ system, the OmniPrep™ for Gram Positive Bacteria kit isolates high quality genomic DNA from Gram-positive bacteria. The kit isolates high purity (A260/A280 ratios of 1.8 to 2.0) DNA (approximately 100kbp) and the yield is 25 to 50μg/ml Gram-positive culture. If used according to protocol, the kit purifies DNA from 100ml Gram-positive bacteria culture.
The kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. Additional LongLife™ Lysozyme is supplied for rapid digestion of bacteria cell walls and DNA release. Pure genomic DNA is isolated in 20 to 40 minutes, depending on the bacteria cell type used.
Features
- High Yield, ~100kb genomic DNA
- Simple two tube method, 20-40 minutes
- No toxic chemicals, no phenol, no hazardous waste
- Supplied with LongLife™ Lysozyme for rapid bacterial cell wall digestion
Applications
- Extraction of pure genomic DNA from Gram negative bacteria
- Extraction of high quality, 100kb genomic DNA