Liberase TL Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a low concentration of Thermolysin, a non-clostridial neutral protease.
- Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
- Count on higher specific activity of the enzyme blend as a result of higher Collagenase I + II purit(determined by HPLC analysis)
- Obtain higher experimental reproducibility due to higher lot-to-lot consistency.
- Increase safety with an enzyme that is free of any mammalian or avian tissue-derived raw materials.
Application
Liberase™ TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold. This product has been used for the isolation of pancreatic islets from mice.
Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25°C.
Note: Avoid repeated freezing and thawing!