M-SAN HQ (Bioprocessing Grade) is the recent addition to our high salt tolerance nucleases portfolio. The biochemical properties of the SAN family of nucleases make them ideal for multiple bioprocessing and biomanufacturing workflows.
M-SAN HQ has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspeci?c endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM.
Properties:
- Source: Recombinantly produced in Pichia pastoris
- Molecular weight: 24.5 kDa
- Protein Purity: > 99% by SDS-PAGE analysis
- Isoelectric point: 8.43
- Unit definition: One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37° in 25 mM Tris-HCl pH 7.6 (@25°C), 2.5 mM MgCl2, 150 mM NaCl, and 50 μg/ml calf thymus DNA.
- Specificity: Nonspecific endonuclease cleaving single and double stranded DNA and RNA.
- Working ranges:
- Temperature: 20 – 50°C, optimal: 36 – 50°C
- Salt concentrations (NaCI): 10 – 500 mM, optimal: 125 mM – 250 mM
- Mg2+: > 0.5 mM is required for activity, optimal: 4 – 15 mM
- pH: 6.5 – 9.5, optimal: 7.2 – 8.7
Note: The working range is defined as above 10% activity and optimal range as above 80% activity.
- Tolerance to typical buffer additives:
- DTT and other reducing agents may inactivate M-SAN HQ
- Urea: Not recommended
- EDTA: Not recommended
*Note: 200kU, 500kU, 1MU, and 5MU sizes available upon request. Contact your Thomas Scientific sales representative today for a quote!