ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
Properties
• Source: Recombinantly produced in Pichia pastoris
- Specific activity: >30 Units/mg
• Size: 34 kDa
• Unit definition: One unit is defined as the amount of enzyme that produces one µmol 4-nitroaniline (extinction coefficient 8.8 mM-1 at 410 nm) at 25°C, pH 8.0 per minute (buffer conditions 50 mM Tris-HCl pH 8.0, 1% DMSO, 1 mM Suc-Ala-Ala-Pro-Phe-pNA).
- Specificity: Nonspecific endopeptidase
- Activity:
- Highly active in temperature range of 25–40°C
- pH: 7–10
- Tolerance to typical buffer additives:
- Inhibitors: The enzyme is inhibited by general serine protease inhibitors, such as PMSF.
- Storage: The enzyme is stable upon storage at -20°C for up to 2 years. ArcticZymes Proteinase FREE is stable upon storage at -20°C for up to 1 year. Additional stability data is available on request.
- Storage buffer:
- 25 mM Tris-HCl, pH 8.0 (25°C)
- 100 mM NaCl
- 50% Glycerol
- Storage buffer (Glycerol FREE):
- 25 mM Tris-HCl, pH 8.0 (25°C)
- 100 mM NaCl
- 1 M Sucrose