This triple antibiotic solution contains penicillin (10,000 IU/mL), streptomycin (10 mg/mL), and amphotericin B (25 μg/mL).
Applications
Penicllin-Streptomycin-Amphotericin B is used to supplement cell culture media. It is effective against yeast, fungi, gram-positive and gram-negative bacteria.
Biochem/physiol Actions
Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out. Antimicrobial spectrum: Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts.
Typical Working Concentration
Recommended working concentration is 10 mg/L