Protein A binds specifically to Fc regions of many mammalian immunoglobulins. Protein A- conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. Protein A-Separopore® 4B-CL is used for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. This is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.