- Evaluate endothelial cell invasion using real-time fluorescence detection in a simplified and reproducible manner
- Increase screening throughput for prospective pro- and anti-angiogenic compounds
During angiogenesis, endothelial cells are activated and express matrix metalloproteinases (MMPs), which degrade the vascular basement membrane and facilitate cellular movement towards angiogenic stimuli. The Corning® BioCoat™ Angiogenesis System: Endothelial Cell Invasion provides an in vitro, quantitative assay for prospective anti- and pro-angiogenic compounds. It is composed of a Corning® Falcon™ 24-Multiwell Insert Plate (and a non-TC-treated 24- or 96-well receiver plate and lid) containing a Corning® FluoroBlok™ fluorescence-blocking microporous (3.0 µm pore size) polyethylene terephthalate (PET) membrane coated with Corning® Matrigel®™ Matrix. Corning® Matrigel® Matrix is a reconstituted basement membrane preparation derived from Engelbreth-Holm-Swarm (EHS) mouse tumors. Furthermore, this matrix is primarily composed of laminin, collagen iv, entactin, and a number of growth factors. An optimized coating process effectively occludes the membrane pores and provides a functional barrier that is analogous to the basement membrane in vivo. This coating blocks the passage of non-invasive cells and allows the passage of activated endothelial cells with invasive capacity. Following activation by angiogenic factors, the endothelial cells express MMP 2 and 9. These proteases digest the matrix and enable the cells to invade through the matrix barrier to the bottom side of the microporous membrane.
Quality Control:
- Tested for its ability to allow invasion of HMVEC-1 cells, an invasive human microvascular endothelial cell line, and to exclude invasion of NIH-3T3 cells, a non-invasive fibroblast cell line.
- Tested and found negative for bacteria and fungi.
Storage and Stability:
- Stable for at least three months from the date of shipping when stored at -20°C.