8.0 ± 0.15, Buffers
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HEPES Buffer (1 M, pH 8.0)
Boston BioProductsComponents: HEPES(1 M), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: 2-8°C
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RIPA Buffer (2X, pH 8.0)
Boston BioProductsComponents: Sodium Dodecyl Sulfate (SDS)(0.2%), Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 (1X) …
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Sodium Phosphate Buffer (0.5 M, pH 8.0)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(500 mM), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.
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EGTA (0.5 M, pH 8.0, Autoclaved)
Boston BioProductsComponents: EGTA(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 8.0 ± 0.15 Storage: Room Temp.
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EDTA (0.5 M, pH 8.0, pH Adjusted with NaOH, Autoclaved)
Boston BioProductsComponents: EDTA(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 8.0 ± 0.15 Storage: Room Temp.
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Tris-EDTA Buffer (pH 8.0, Low Endotoxin)
Boston BioProductsComponents: Tris Base / Tris-HCl(10 mM), EDTA(1 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.
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HEPPS Buffer (0.2 M, pH 8.0)
Boston BioProductsComponents: HEPPS (EPPS)(200 mM), Sodium Hydroxide(For pH adjustment), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: 2-8°C
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Tris-EDTA Buffer (20X, pH 8.0, Autoclaved)
Boston BioProductsComponents: Tris Base / Tris-HCl(200 mM), EDTA Disodium Dihydrate(20 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 8.0 ± 0.15 (1X) Storage: Room Temp.
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Bicine Buffer (0.5 M, pH 8.0)
Boston BioProductsComponents: Bicine(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.
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RIPA Buffer (pH 8.0)
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Nonidet P-40 Substitute(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 …
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HEPES Buffer (0.5 M, pH 8.0)
Boston BioProductsComponents: HEPES(500 mM), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: 2-8°C
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Tris-Acetate-EDTA (10X)
Boston BioProductsComponents: Tris-Base(400 mM), Acetic Acid(200 mM), EDTA Disodium Dihydrate(10 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 8.0 ± 0.15 (1X) Storage: Room Temp.
