5 mL, bioWORLD, Affinity Chromatography

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  • Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…

  • Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…

  • Supplied as a 2.5% suspension MagneZoom™-Epoxy are silica-coated super paramagnetic beads with an Epoxy surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads. Magnetic bead ligand: Epoxy. Particle size:…

  • Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • bioSilica Beads Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, a density of 1.96g/cm 3 , a refractive index (589nm) of about 1.37. Silica beads have high temperature stability. Also, because hydrophilic silica does not absorb proteins,…

  • MagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…

  • Recombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants. Particle size: 2-5µm Ligand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beads pH stability: 4-9 …

  • Wheat germ lectin (Isolated from Triticum vulgaris) conjugated to BlackPearl® Magnetic Beads. Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor…

  • Recombinant protein G covalently linked to MagneZoom (paramagnetic beads). Protein G binds Rc region of mammalian immunoglobulin. Ideal for purification and fractionation of IgG subclasses that poorly bind protein A. Suitable for high throughput screening. Particle size: 2-5µm Ligand:…

  • Zerobind™ affinity columns are designed to remove non-specific backgrounds from various polyclonal antibodies generally used for western blotting. Passing the diluted Antibody solution through Zerobind™ column can reduce non-specific binding while retaining activity against your…

  • Wheat germ -Triticum vulgaris- agglutinin (WGA)  linked to MagneZoom (magnetic beads). For magnetic separation by binding specifically to cell particles, glycoconjugates and polysachharides containing N-acetylglucosamine residues. Size: 2-5µm. Ligand: Wheat germ agglutinin  Ligand…

  • Erythrina cristagalli (coral tree) lectin is a 54,000 dalton glycoprotein consisting of two different subunits of approximately 28,000 and 26,000 daltons. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl ( b -1,4)…

  • Silica-coated uniform super paramagnetic beads with surface carboxyl group. Proteins and nucleic acids can be covalently bound to these beads with the retention of biological activity. Size: 5-10µm. COOH terminated Binding: up to 20mg/ml. pH stability: 4-9 Supplied as 25mg/ml…

  • Supplied as a 2.5% suspension MagneZoom™-COOH are silica-coated super paramagnetic beads with an COOH surface group. bioWORLD’s MagneTons are suitable for high throughput screening Matrix: Silica coated magnetic beads. Magnetic bead ligand: COOH. Particle size: 1-2µm. …

  • Supplied as a 2.5% suspension. MagneZoom™-NH2 are silica-coated super paramagnetic beads with an NH2 surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads Magnetic bead ligand: NH2 Particle size: 1-2µm …

  • Applications: Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to this immobilized ligand. The term "Thiophilic" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic adsorption…

  • Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

  • A complete kit to bind Protein A fusion proteins expressed in E. coli or yeast. Kit consists of affinity chromatography gel matrix, IgG-Separopore® 4B, including all the components/buffers that allow for quick, convenient purification of anti-IgG antibodies. This kit may also be used to…

  • Application: Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.

  • Streptavidin covalently linked to MagneZoom™ (magnetic beads). For magnetic separation and isolation of biotinylated ligands for affinity separations. Used in high throughput screening. Surface silica coated magnetic beads Bead Size: 3-5 µm Supplied as suspension (25mg/ml) in phosphate…

  • Applications: The red-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows…

  • For isolation and purification of pure and intact mRNA from crude extract of animals and plant tissues. It is supplied as a suspension of super paramagnetic beads covalently coupled to Oligo (dT)25. Technical specification: Matrix: Silica coated Magnetic beads Ligand: Oligo (dT)25 …

  • Applications: Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in…

  • Jacalin, α D-galactose-specific lectin from jackfruit, interacts with human IgA and one or two other serum proteins. Jacalin coupled Separpore ® is used for the purification of glycoproteins containing α-D-galactose. bio-World provides Jacalin-Separpore ® as a suspension…

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