3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a…

Ac-DEVD-AFC is a sensitive fluorescent substrate for the assay of caspase-3 activity. The trifluoromethyl substituent of the fluorophore improves the membrane permeability of the substrate.

Isoleucine provides a source of nitrogen for transport to the liver and kidney.

Lithium Bromide

3,3-Diaminobenzidine Tetrahydrochloride is a reagent used in colorimetric analysis of Se and other metals. DAB is utilized in many applications for visualization of peroxidase activity. In the peroxidase reaction DAB serves as a hydrogen donor in the presence of peroxide; the oxidized DAB forms…

8-Anilino-1-naphthalene Sulfonic Acid is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle…

Sodium Acetate is used as a buffer in the buffering range of pH 3.6-5.6.

Ammonium persulfate is a strong oxidizing agent. It is a radical initiator. Ammonium persulfate is a polymerization catalyst used with TEMED for polyacrylamide gel formation. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry. Other…

Hydrogen peroxide is used as a reagent, disinfectant, antiseptic, and propellant. In synthetic chemistry, H2O2 is used for epoxidation and hydroxylation transformations, and is used as the raw oxidant source for generating more complicated and specific organic/inorganic peroxides.

PMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.

Resorcinol is an aromatic alcohol used in large scale processes such as tanning, the manufacture of resins and resin adhesives, the production of hexylresorcinol and p-aminosalicylic acid, and the dyeing and printing of textiles.

Transfer Ribonucleic Acid can be used for a tRNA standard and in reactions involving tRNAs, such as assays for tRNA synthetase activity.

Ninhydrin is used for the detection of free amino groups in amino acids, peptides and proteins and in assay of peptides, amino acids, amino sugars and amines. It is commonly used for the development of fingermarks on porous surfaces and is often used in comparison with other latent fingerprint…

Azure B Certified is used for differentiating cellular RNA (purplish) and DNA (blue-green) in plant tissues. Useful as a stain for Negri Bodies. Can be used in the Romanowsky-type stains for blood and blood protozoa.

Adenosine-5'-triphosphate (ATP) disodium salt, >99%. Crystalline.

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in…

Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus niger. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Unit Definition:…

PMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.

Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutanyl amino acids and reductive cleavage of disulfide bonds.

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates.

Tetracyline inhibits gram-positive, gram-negative and mycoplasma. It is used in tetracycline controlled gene expression systems (gene switches) such as the tet-on and tet-off systems. It is also used to select tetracyline resistant colonies in cell culture.

Oleoyl Coenzyme A Dipotassium Salt

Cholesteryl Hydrogen Succinate

Bromocresol Green is a dye of the triphenylmethane family (triarylmethane dyes). It binds quantitatively with human albumin forming an intense blue-green complex with an absorbance maximum at 628 nm. Bromocresol Green is useful in a variety of colorimetric detection technologies. It is used as a…

In the research labs it is used as an astringent, antiseptic, antibacterial and antifungal agent. Used in buffers and for calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis.

Sequestrant for metal ions.

N-Acetyl-ß-D-mannosamine hydrate is used as a substrate to identify, differentiate and characterize enzymes such as N-acetyl-glucosamine epimerase(s) and aldolase(s). N-Acetyl-D-mannosamine is used for the synthesis of of N-acetylneuraminic acid.

Coomassie® Brilliant Blue R-250 is a fast acting, sensitive dye. Brilliant Blue R can be used for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. Other applications for…

Cholic acid is used in the solubilization of membrane-bound proteins in their native state. It is a component for the preparation of liposomes. It has also been used in a study to investigate the elastic deformable vesicles, transfersomes, for transdermal drug delivery.

Gluteraldehyde is a protein cross-linking agent. Also possesses sporicidal activity. This product is also suitable for use as an electron microscopy fixative. Glutaraldehyde reacts through cross-linking to impart water resistance to protein and polyhydroxy compounds. It is also a reducing agent for photochemicals.

Lactic acid is an a hydroxyl carboxylic acid produced from pyruvate by the enzyme lactate dehydrogenase (LDH). Lactate is one of the main components of Ringer's lactate or lactated Ringer's solution. It is a fermentation acid from milk or carbohydrates

Glucose-6-phosphate dehydrogenase (G-6-PDH) was used as a model to test the effect of seed protein fractions on enzyme protection during dehydration. G-6-PDH has been utilized in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.