LGC, Biosearch Technologies, Reagents, Reagents, P
-
TRANSFORMAX EPI300 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically competent E. coli engineered to regulate the copy number of CopyControl Fosmid clones.
-
EconoTaq PLUS
LGC, Biosearch TechnologiesEconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR. More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify…
-
NxSeq HT Dual Indexing Kit (96 Reactions)
LGC, Biosearch TechnologiesThe NxSeq HT Dual Indexing Kit is required for the construction of DNA fragment libraries using the NxSeq UltraLow Kit, 96 Reactions. This kit provides the Universal Adaptor and Dual Indexing Primers required for the construction of 96 libraries with unique dual indices.
-
LavaLAMP DNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…
-
Ampliscribe T7-Flash Transcription Kits
LGC, Biosearch TechnologiesThe AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.
-
TG1 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…
-
SS320 (MC1061 F) Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
-
Bst DNA Polymerase
LGC, Biosearch TechnologiesBst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription…
-
MC1061 F- Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
-
E. cloni EXPRESS BL21(DE3) Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…
-
NxSeq AmpFREE Low DNA Library Kits
LGC, Biosearch TechnologiesThe NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours…
-
Hybridase Thermostable RNase H 500 U
LGC, Biosearch TechnologiesHybridase™ Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA.
-
T4 Polynucleotide Kinase, Cloned 3,000 U
LGC, Biosearch TechnologiesT4 Polynucleotide Kinase (PNK) catalyzes the transfer of the ?-phosphate from ATP to the 5´ hydroxyl of ssDNA and dsDNA, RNA, and nucleoside 3´ monophosphates.
-
COPYCUTTER INDUCTION SOLUTION 25 ml
LGC, Biosearch TechnologiesPrepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.
-
TRANSFORMAX EC100 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.
-
OverExpress C41 (DE3) Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…
-
TE Buffer 70 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
-
NxGen phi29 DNA Polymerase
LGC, Biosearch Technologiesphi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
-
Plasmid-Safe ATP-Dependent DNase
LGC, Biosearch TechnologiesPlasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
-
E. cloni 10G 96-well Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of…
-
NxGen RNAse Inhibitor
LGC, Biosearch TechnologiesRNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.
-
Ribonuclease R (RNase R) 250 U
LGC, Biosearch TechnologiesRibonuclease R (RNase R) from E. coli is a magnesium-dependent 3´′→5´ exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures.
-
Baseline-ZERO DNase 5,000 U
LGC, Biosearch TechnologiesBaseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
-
RNase A 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
-
MMLV HIGH PERFORMANCE REV TRANS 25,000 U
LGC, Biosearch TechnologiesThe MMLV High Performance Reverse Transcriptase produces full-length first-strand cDNA from total RNA preparations or purified poly(A+) RNA.
-
TRANSFORMAX EC100 ELECTROCOMP E. COLI 20 rxns
LGC, Biosearch TechnologiesTransforMax™ EC100™ Electrocompetent E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency.
-
RNA 5' Polyphosphatase 200 U
LGC, Biosearch TechnologiesRNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi
-
RNase-Free DNase I
LGC, Biosearch TechnologiesRNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.
-
MMLV REVERSE TRANS 1ST-STRD CDNA SYN KIT 50 rxns
LGC, Biosearch TechnologiesThe MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit is optimized for generating full-length first-strand cDNA from total RNA preparations or purified Poly(A)+ RNA.
-
MasterAmp 10X PCR Enhancer 10 ml
LGC, Biosearch TechnologiesFor use with the MasterAmp™ PCR Optimization Kits.
-
Proteinase K 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
-
Ready-Lyse Lysozyme Solution
LGC, Biosearch TechnologiesReady-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.