Ponceau S is a stain used to visualize proteins on nitrocellulose of PVDF membranes. To create Ponceau S solution; dissolve 0.5gm of Ponceau S in 1ml of glacial acetic acid, and bring to 100ml with ddi water. To stain with Ponceau S; remove blot and rinse with ddi water, stain with Ponceau S…