HiMedia, Supplies, Tissue Culture Media
HIMEDIA
State-of-the-Art products in microbiology,cell culture, plant tissue culture, and molecular biology.
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Tryptose Phosphate Broth 50X
HiMediaTryptose Phosphate Broth 50X. Used to enhance the growth supporting property of GMEM for BHK-21 cells. It is also used in insect cell culture media to support the growth of SF-21 cells.
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Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, L-Glutamine, NEAA and 2.2gms per litre Sodium bicarbonate. As per ATCC composition
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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ZymeFree™ Enzyme Free Cell Dissociation Reagent in Dulbecco’s Phosphate Buffered Saline 1X. Chemically defined cell dissociation reagent used for gentle dissociation of adherent cells.
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Hanks' Balanced Salt Solution 1X w/ Phenol red and Sodium bicarbonate w/o Calcium. Used with cells maintained in less CO2 environment or CO2 free environment.
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Water, Sterile, Molecular Biology Grade Endotoxin tested, DEPC treated, Nuclease and Protease free. Used for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA.
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1X PBS is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced…
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Dulbecco's Phosphate Buffered Saline 1X w/o Phenol red, Calcium and Magnesium. Commonly used for tissue disaggregation and monolayer dispersal.
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Uridine is a ribonucleoside that has Uracil attached to its ribose ring. It is an important component of carbohydrate metabolism and is essential for both RNA and DNA synthesis. The monophosphate form of uridine (UMP) is formed from orotidylic acid (orotidine 5'- monophosphate) in a decarboxylation…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
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McCoy's 5A Medium
HiMediaMcCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to…
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Phosphate Buffered Saline, pH 7.2 10X. Commonly used for tissue disaggregation and monolayer dispersal.
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The HiMedia water/sterile/tissue culture grade endotoxin tested is mainly used in research facilities and chemical laboratories to conduct wide range of experiments. This DEPC treated compound is nuclease and protease free. It is available in sturdy bottle that endures heavy and rigorous usage.
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Hanks' Balanced Salt Solution 10X w/ Phenol red w/o Sodium bicarbonate. Used with cells maintained in less CO2 environment or CO2 free environment.
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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Technical Data Sheet
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10X PBS used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt…
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Dulbecco's Phosphate Buffered Saline is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a…
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Sodium bicarbonate Solution 7.5%
HiMediaSodium bicarbonate Solution 7.5%. Used as a biological buffer in cell culture media to maintain physiological pH.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Sodium Pyruvate Solution 100mM
HiMediaSodium Pyruvate Solution 100mM. Used as a medium supplement for energy source and a carbon skeleton for anabolic processes.
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CMRL media are based on the composition of Medium 199. Nucleic acid derivatives, coenzymes omitted in H597 modification were retained in CMRL 1066. CMRL 1066 is a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. Although…
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HEPES 1M Solution
HiMediaHEPES 1M Solution. Used in cell culture media to maintain the pH levels of basal media.
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Gelatin Solution 2% w/ 2% Type B Gelatin from bovine skin in sterile tissue culture grade water. Used in cell culture for coating the cell culture plates and flasks to improve the cell attachment for certain types of cells.
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer. Contains stabililzed dipeptide of L-Glutamine.
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HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37deg C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which…
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Water, Sterile, Tissue Culture Grade. Used for reconstitution of powdered media and other cell culture reagents.
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20X PBS used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt…
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Phenol red 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used as a pH indicator in cell culture media.
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TCL016 is molecular biology grade sterile filtered water, treated with 0.1% Diethylpyrocarbonate(DEPC). It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionization, and filtration. It is produced specifically for molecular biology…