Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue,…

A fluorescent substrate for b-D-Glucuronidase (GUS) encoded by the gusA gene isolated originally from E.coli. Cleavage of the substrate 4-MUG by a b-glucuronidase activity leads to the generation of the fluorigenic product 4-MU, which can be visualized or detected by irradiation with UV light. The…

Also known as 5-Bromo-4-Chloro-3-indolyl-β-D-Glucopyranoside. A substrate for Beta-glucosidase that renders an intense indigo-blue chromophore (615 nm) upon enzymatic action. Used as an indicatior-probe in histochemistry and in culture media to detect beta-Glucosidase positive organisms.