MilliporeSigma, UN 3245, Reagents
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MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-210-3p
MilliporeSigmaIndividual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a…
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MISSION(R) TRC3 ORF BFP Lentivirus Control
MilliporeSigmaThe MISSION TRC3 ORF BFP Lentivirus Control Transduction Particles contain an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF…
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Sigma(R) is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC.The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG…
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Developed by Feng Zhang's lab at the Broad Institute, the mouse GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…
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MISSION(R) Lenti microRNA, Human hsa-miR-95-3p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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MDR1 Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 (MDR1) efflux transporter. Synonyms: C2BBe1 Cells MDR1 (-/-/-/-) …
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Kidney PTEC Control Cell (SA7K Clone)
MilliporeSigmaSigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma's RPTEC's exhibit normal epithelial…
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SIG10 ULTRA Electrocompetent Cells for BAC & cosmid cloning
MilliporeSigmaThe SIG10 ULTRA Electrocompetent Cells have the highest transformation efficiency available. They are to be used for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants…
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The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types…
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MISSION(R) TRC3 ORF GFP Lentivirus High Titer Control
MilliporeSigmaThe MISSION TRC3 ORF GFP Lentivirus High Titer Control Transduction Particles contain an ORF insert expressing TurboGFP(TM), and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole…
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MISSION(R) TRC3 ORF GFP Lentivirus Control
MilliporeSigmaThe MISSION TRC3 ORF GFP Lentivirus Control Transduction Particles contain an ORF insert expressing TurboGFP(TM), and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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The transcriptional activator VP64 fused to nuclease-dead SpCas9 (dCas9), forms a ribonucleoprotein complex (RNP) with the CRISPR guide RNA (gRNA). The stem- and tetra-loop sequences in the gRNA scaffold have been modified into minimal hairpin RNA aptamers, which selectively bind dimerized MS2…
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XLDNA SIG10 Electrocompetent cells for BAC & cosmid cloning
MilliporeSigmaThe XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields. These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct…
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Developed by Feng Zhang's lab at the Broad Institute, the human GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the human genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…
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MISSION(R) Lenti microRNA, Human hsa-miR-146a-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
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MRP2 Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC2 (MRP2) efflux transporter. Storage: -196C Application: The…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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OverExpress(TM) Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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BCRP Knockout Caco-2 Cells one vial
MilliporeSigmaThe C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCG2 (BCRP) efflux transporter. Synonyms: C2BBe1 Cells BCRP (-/-/-/-) …
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MISSION(R) Lenti microRNA, Human hsa-miR-342-5p
MilliporeSigmaSigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the…
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Cas9 Hygromycin Lentiviral Particles
MilliporeSigmaReady-to-use Cas9-Hygro lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-Hygro lentiviral particles efficiently and stably integrate Cas9 and a hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-Hygro) for…
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When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…
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Ready to use MS2-P65-HSF1 lentiviral particles enable immediate transduction of a wide range of cell lines. MS2-P65-HSF1 lentiviral particles efficiently and stably integrate MS2-P65-HSF1 and hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter…
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OverExpress(TM) Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been…
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Cas9 RFP Lentiviral Particles
MilliporeSigmaReady-to-use Cas9-RFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-RFP lentiviral particles efficiently and stably integrate Cas9 and RFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing…
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The Lenti CRISPR Human HPRT1 Positive Control Transduction Particles (LV04 vector) product is a critical positive control to monitor transduction efficiency for lentiCRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human HPRT1. The protein encoded by…
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MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-122-5p
MilliporeSigmaIndividual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a…