Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation, while inhibiting gram-positive bacteria (1). Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo was successful in inhibiting gram-positive…

CAS No.: 7558-80-7 Molecular Formula: NaH₂PO₄ Molecular Weight: 119.98

XLD Agar was formulated by Taylor (1-5) for the isolation and differentiation of enteric pathogens including Salmonella Typhi from other Salmonella species. XLD Agar has been recommended for the identification of Enterobacteriaceae (7) and for the microbiological testing of foods, water…

Peptone Water is particularly suitable as a substrate in the study of indole production. Peptic digest of animal tissue used in Peptone Water is rich in tryptophan content. Peptone Water is also utilized as a base for carbohydrate fermentation studies with the addition of sugar and indicators such…

Deoxycholate Agar is prepared as per the formulation by Leifson (1). This media is used for the isolation and maximum recovery of intestinal pathogens belonging to Salmonella and Shigella species (2). The selectivity of medium permits the use of fairly heavy inocula without danger of overgrowth…

Peptone Water is recommended by BIS (1) for detection of Indole production by Escherichia coli . A slightly different formulation of Peptone water/ Peptone water with phenol red is recommended (2,3,4) for studying the ability of organisms to ferment a specific carbohydrate which aids in…

Lactose is a disaccharide. It is a sugar composed of galactose and glucose subunits and has the molecular formula C12H22O11. Lactose makes up around 2-8% of milk (by weight).

Endo (1) had first developed a culture medium for differentiation of lactose fermentors and non-fermenters and further developed as todays Endo Agar (2). Endo agar is used for microbiological examination of potable water and waste water, dairy products and food (3,4,5). Sodium sulphite and…

Glycerol is odorless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. Application:…

Polyoxyethylenesorbitan monolaurate commonly known as Tween 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty…