Cellomics Technology
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CMV-LHX2 lentivirus
Cellomics TechnologyLHX2 (LIM Homeobox 2) belongs to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. LHX2 is involved in neuronal differentiation. This lentivirus expresses human LHX2 under the control of the CMV promoter.
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Inducible tet-on MITF-D lentivirus (Bsd)
Cellomics TechnologyMicrophthalmia-associated transcription factor (MITF) is a member of the basic/helix-loop-helix/leucine zipper (b-HLH-ZIP) transcription factor subfamily. MITF regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived…
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EF1A-C-MYC lentivirus
Cellomics TechnologyMYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin…
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Inducible tet-on NEUROD1 lentivirus
Cellomics TechnologyNEUROD1 is a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and…
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ADAM-17 shRNA lentivirus
Cellomics TechnologyADAM-17 gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM-17 is an…
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PGK-Cre, CMV-Renilla luciferase lentivirus
Cellomics TechnologyCre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…
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Inducible tet-on NR2E3 lentivirus
Cellomics TechnologyNR2E3, a photoreceptor-specific orphan nuclear receptor, is believed to play a pivotal role in the differentiation of photoreceptors. Mutations in NR2E3 result in syndromes characterized by excess blue cones and loss of rods: enhanced S-cone syndrome (ESCS). This lentivirus expresses human NR2E3…
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Set of lentivirus (EF1A-driven LIN28, NANOG, SOX2, OCT3/4, KLF4 and C-MYC)
Cellomics TechnologyThis set contains six EF1A-driven vectors: LIN28 (PLV-10015), NANOG (PLV-10014), SOX2 (PLV-10013), OCT3/4 (PLV-10012), KLF4 (PLV-10011) and C-MYC (PLV-10010). Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in…
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Calcium indicator GCaMP3 lentivirus (CMV)
Cellomics TechnologyCalcium is a universal second messenger regulating essential cellular signaling events in a broad range of tissues and organisms. GCaMP3 is a fusion calcium indicator of green fluorescent protein (GFP), calmodulin and the M13 domain of a myosin light chain kinase designed to show the calcium ion…
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Inducible tet-on PAX6 lentivirus
Cellomics TechnologyPax6 gene contains a paired domain and a paired-type homeodomain and is expressed during early neuroretina development. Pax6 is required for multipotency of retinal progenitors. Tansfection of Pax6 into ES or iPS cells induces retinal progenitors. Pax6 alone is sufficient to induce…
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Inducible tet-on MITF-D lentivirus (Puro)
Cellomics TechnologyMicrophthalmia-associated transcription factor (MITF) is a member of the basic/helix-loop-helix/leucine zipper (b-HLH-ZIP) transcription factor subfamily. MITF regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived…
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PGK-GFP lentivirus
Cellomics TechnologyGreen fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…
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Angiogenin (Ang) shRNA lentivirus
Cellomics TechnologyAngiogenin (Ang) gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is…
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CMV-SOX2 lentivirus
Cellomics TechnologySRY (sex determining region Y)-box 2 (SOX2) is a transcription factor critical for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of SOX2 has been used to produce iPS cells in culture.
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CMV-TNFSF12 lentivirus
Cellomics TechnologyTNFSF12 is a member of the tumor necrosis factor (TNF) ligand family. TNFSF12 is a ligand for the FN14/TWEAKER receptor involved in apoptosis and angiogenesis. This lentivirus expresses human TNFSF12 under the control of the CMV promoter.
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CMV-LacZ lentivirus
Cellomics TechnologyLacZ catalyzes the hydrolysis of the disaccharide lactose, into gluclose and galactose. The use of LacZ as a reporter gene is due to its ability to cleave the bond between galactose and the indole, 5-bromo-4-chloro-3-hydroxyindole. The cleaved indole, is an easily detectable and insoluble blue compound.
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Inducible tet-on SOX2 lentivirus
Cellomics TechnologySRY (sex determining region Y)-box 2 (SOX2) is a transcription factor critical for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of SOX2 has been used to produce iPS cells in culture. This lentivirus expresses Sox2 under the control of the Tet promoter.
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CMV-OSKM lentivirus
Cellomics TechnologyInduced pluripotent stem (iPS) cells can be generated from differentiated somatic cells by over-expressing a set of specific transcription factors called reprogramming factors. Typically four reprogramming factors are chosen, Oct4, Sox2, Klf4 and c-Myc (OSKM), which are introduced into the target…
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NF-kB Firefly luciferase reporter lentivirus (Puro)
Cellomics TechnologyThis NF-kB reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the NF-kB transcriptional response element (TRE). It can be used to monitor the transcriptional activity of NF-kB.
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CMV-GFP-IRES-Puro lentivirus
Cellomics TechnologyGreen fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…
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EF1A-LIN28 lentivirus
Cellomics TechnologyLIN28 is a microRNA-binding protein that has been shown to bind the let-7 pre-microRNA and block production of mature let-7 in embryonic stem cells. This activity appears to preserve undifferentiated state of stem cells. Subsequently, LIN28 expression has been used to generate iPS cells. Human…
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CAG-RFP lentivirus
Cellomics TechnologyTurboRFP is a red (orange) fluorescent protein (ex. 553 and em. 574 nm) derived from sea anemoneEntacmaea quadricolor [Merzlyak et al., 2007]. Possessing high photostability and pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in…
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BMP4 shRNA lentivirus
Cellomics TechnologyBMP4 gene encodes a protein of the bone morphogenetic protein (BMP) family, which is part of the transforming growth factor-beta (TGF-beta) superfamily. The superfamily includes large families of growth and differentiation factors. Members of the BMP family play an important role in the onset of…
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EF1A-OCT3/4 lentivirus
Cellomics TechnologyOCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…
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CMV-MITF-H lentivirus
Cellomics TechnologyMITF-H is an isoform of MITF (microphthalmia-associated transcription factor), a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible…
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CMV-Cre-GFP lentivirus
Cellomics TechnologyCre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…
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CMV-Pax6 lentivirus
Cellomics TechnologyPax6 (paired box 6) is a transcriptional regulator with paired box domain and homeo box domain. Pax6 is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. This lentivirus expresses…
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Set of lentivirus (Tet-driven SOX2, OCT3/4, KLF4 and C-MYC)
Cellomics TechnologyThis set contains four Tet-driven vectors: KLF4 (PLV-10026), SOX2 (PLV-10027), C-MYC (PLV-10028), and OCT3/4 (PLV-10019).
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Inducible tet-on CRX lentivirus
Cellomics TechnologyCRX is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. CRX play an important role in transdifferentiation of photoreceptor cells. This lentivirus expresses human Crx under the control of the Tet promoter.
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Inducible tet-on Brn2 lentivirus
Cellomics TechnologyBRN2 (also known as POU3F2) encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an…
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Inducible tet-on Smad6 lentivirus
Cellomics TechnologySmad6 and Smad7 are inhibitory Smads induced by transforming growth factor β-Smad signal transduction pathways in a negative-feedback mechanism. Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube. Smad6 is also involved in multiple tissue differentiation…
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Inducible tet-on OSKM lentivirus
Cellomics TechnologyInduced pluripotent stem (iPS) cells can be generated from differentiated somatic cells by over-expressing a set of specific transcription factors called reprogramming factors. Typically four reprogramming factors are chosen, Oct4, Sox2, Klf4 and c-Myc (OSKM), which are introduced into the target…