Application: L-Phenylalanine-Separopore® 4B-CL is used for the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase.

Quantitative Analysis:  Protein extract from E. coli containing a plasmid that expresses a maltose-binding protein-β-galactosidase fusion protein (100,000 units) is passed over a 1 ml column at 4°C. The column is then washed with 10 column volumes of 20 mM Tris-HCl (pH 7.4), 0.2 M…

Applications : L-Aspartic Acid-Separopore® 4B-CL is used for the purification of retinol, or other L-Aspartic Acid binding proteins.

Application: L-Lysine-Separopore® 4B-CL is used for purification of molecules with biospecific or charge dependent affinity for L-lysine. It is also used for the purification of lysine-binding proteins from serum and to purify plasminogen, plasminogen activator, ribosomal RNA, and…

Application: For the purification of membrane dicarboxylate binding proteins and other proteins with affinity to glutamate.

Applications: Histidine-Separopore® 4B-CL can be used in the purification of supercoiled (sc) plasmid DNA (pDNA) from Escherichia coli lysates. This can also be used for the separation of IgG from human plasma and selective purification of bioactive oligouronides such as α-(1,…