Nutritionally rich media designed for growth of pure cultures of recombinant strains. 10 g/L of salt formulation Autoclave sterilized LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular…

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…

Calcium Chloride 2 M is useful in the preparation of competent E.coli and transformation of bacteria. Preparation of competent E.coli Transformation of bacteria 2 Molar concentration Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease tested Applications Calcium chloride is…

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H. Used with SOC medium Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure,…