Sheilds and Sang M3 medium is based on the original formula of D22 medium. The medium supports the growth of the cells derived from Drosophila melanogaster. Sheilds and Sang (1977) eliminated chloride salts present in the previous formulation and introduced potassium and sodium ions in the form of…

Glasgow's Minimum Essential Medium is a modification of Basal Medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells, used for investigating…

Grace's medium is a modification of the original formula developed by Wyatt. Grace (1962) improved the medium by introducing ten water soluble vitamins in Wyatt's formula. Grace successfully established four strains of cells from ovarian tissue of Australian emperor gum moth using this medium.…

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely…

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely…

Mitsuhashi and Maramorosch Insect Medium is specially used for the growth and propogation of mosquito cell lines especially Aedes aegypticus . It was originally developed to grow cells derived from Leafhopper. Subsequently, it has been used to culture cells derived from a number of insect species.…

Basal Medium developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. The medium was initially developed as a result of studies to determine the nutritional requirements of HeLa cells…

TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV…

Drosophila diet, ID001 is a modification of E. B. Lewis's original formula for fruit fly larvae. This medium mimics the insect's normal food source and provides the necessary nutrition for growth and development of fruit fly larvae. Appearance of Powder: Wheat brown colored granular powder. …

Basal Medium developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. The medium was initially developed as a result of studies to determine the nutritional requirements of HeLa cells…