HiMedia Tissue Culture Media
HIMEDIA
State-of-the-Art products in microbiology,cell culture, plant tissue culture, and molecular biology.
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McCoy's 5A Medium
HiMediaMcCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to…
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Dulbecco's Phosphate Buffered Saline 1X w/ Calcium and Magnesium w/o Phenol red. Commonly used for tissue disaggregation and monolayer dispersal.
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TCL016 is molecular biology grade sterile filtered water, treated with 0.1% Diethylpyrocarbonate(DEPC). It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionization, and filtration. It is produced specifically for molecular biology…
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ZymeFree™ is cell dissociation reagent in Dulbecco's Phosphate buffered saline. It is used for gentle removal of adherent cells from each other and from the surface of a culture vessel. This enzyme free formulation helps in preserving the structural and functional integrity of the cell surface…
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer. Contains stabililzed dipeptide of L-Glutamine.
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Dulbecco's Phosphate Buffered Saline is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a…
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Phosphate Buffered Saline, pH 7.2 10X. Commonly used for tissue disaggregation and monolayer dispersal.
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Water, Sterile, Molecular Biology Grade Endotoxin tested, DEPC treated, Nuclease and Protease free. Used for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA.
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Hanks' Balanced Salt Solution 10X w/ Phenol red w/o Sodium bicarbonate. Used with cells maintained in less CO2 environment or CO2 free environment.
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Phosphate Buffered Saline, pH 7.2 1X. Commonly used for tissue disaggregation and monolayer dispersal.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Phenol red 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used as a pH indicator in cell culture media.
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Sodium Pyruvate Solution 100mM
HiMediaSodium Pyruvate Solution 100mM. Used as a medium supplement for energy source and a carbon skeleton for anabolic processes.
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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1X PBS is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced…
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Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Alanyl-L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. Contains stabililzed dipeptide of L-Glutamine.
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ZymeFree™ Enzyme Free Cell Dissociation Reagent in Dulbecco’s Phosphate Buffered Saline 1X. Chemically defined cell dissociation reagent used for gentle dissociation of adherent cells.
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Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, L-Glutamine, NEAA and 2.2gms per litre Sodium bicarbonate. As per ATCC composition
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Water, Sterile, Tissue Culture Grade. Used for reconstitution of powdered media and other cell culture reagents.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Trypan blue 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used for live-dead cell counting.
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HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37deg C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which…
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Sodium bicarbonate Solution 7.5%
HiMediaSodium bicarbonate Solution 7.5%. Used as a biological buffer in cell culture media to maintain physiological pH.
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CMRL media are based on the composition of Medium 199. Nucleic acid derivatives, coenzymes omitted in H597 modification were retained in CMRL 1066. CMRL 1066 is a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. Although…
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements. Contains stabililzed dipeptide of L-Glutamine.
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Gelatin Solution 2% w/ 2% Type B Gelatin from bovine skin in sterile tissue culture grade water. Used in cell culture for coating the cell culture plates and flasks to improve the cell attachment for certain types of cells.
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Yeast Extract
HiMediaYeast Extract is the water-soluble portion of autolyzed yeast. It provides naturally occurring B-complex vitamins, nitrogen, amino acids and carbon in cell culture media. TCL041 is a sterile filtered solution of Yeast Extract. It is most commonly used as an enrichment supplement to grow Mycoplasma.
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Hanks' Balanced Salt Solution 1X w/ Phenol red and Sodium bicarbonate w/o Calcium. Used with cells maintained in less CO2 environment or CO2 free environment.
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…