Safranin O is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin O has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin O is used to stain for chromosomes. In late Palaeozoic pteridosperm…

Acid fast bacillus stain uses carbol fucshin dye (Ziehl Neelsen method) for identification of acid fast organism.