Low potential for eliciting an immune response, ensuring that the primary response is to the antigen-carrier protein conjugate and not against a determinant on the crosslinker. Less immunogenic than SMCC EReactive groups: NHS ester and maleimide Reactive toward: amino and sulfhydryl groups

Heterobifunctional Reactive groups maleimide and NHS ester Primary amine and sulhydryl reactive Hapten-carrier protein conjugation 1,2 Soluble in DMSO and chloroform Storage: Shipped at ambient temperature, upon receipt store at 4°C.

A popular non-cleavable, membrane-impermeable, water-soluble DSS analog Category: Amine-reactive Reactive Groups: sulfo-NHS-ester Reactive Toward: amino grops Water Soluble: Yes Membrane Permeable: No Cleavability: Non-cleavable MW: 572.43

SMCC, Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate Primary amine and sulfhydryl reactive Enzyme-antibody conjugation 1,2

SPDP, N-Succinimidyl-3-(2-pyridyldithio) Propionate Primary amine and sulfhydryl reactive Thiolation Cleavable

Sulfo-GMBS, N-Maleimidobutryloxysulfosuccinimide ester Primary amine reactive Sulfhydryl reactive M.W. 328.24

Sulfo SMPB, Sulfosuccinimidyl 4-(p-maleimidophenyl) Butyrate Primary amine and sulfhydryl reactive Spacer arm lenght: 11.6A° Water soluble

SIAB, N-Succinimidyl (4-iodoacetyl) Aminobenzoate Primary amine and sulfhydryl reactive Enzyme-antibody conjugation

Sulfo NHS LC SPDP, Sulfosuccinimidyl 6-[3-(2-pyridyldithio) propionamido] Hexanoate Water soluble Spacer arm SPDP Antibody-toxin conjugation

Sulfo-SMCC (Sulfosuccinimidyl-4-N-maleimidomethyl) cyclohexane-1-carboxylate) Synonym: 4-9N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium Primary amine and sulfydryl reactive Enzyme-antibody conjugation Stable maleimide group

SMPB, Succinimidyl-4-(p-maleimidophenyl) Butyrate Primary amine and sulfhydryl reactive Enzyme-antibody conjugation Spacer arm

NHS LC SPDP, Succinimidyl 6-[3-(2-pyridylthio) propionamido] Hexanoate Spacer arm SPDP Antibody-toxin conjugation

m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester, Heterobifunctional Useful for coupling proteins, enzymes to antibodies, toxins to antibodies, and haptens to carrier proteins Category: Amine- and Sulfhydryl- Reactive Reactive Groups: sulfo-NHS-ester and maleimide Reactive…