a-factor is one of the two mating pheromones in baking yeast. It is the “opposite” sex of mating pheromone a-Factor (alpha-factor). When yeast a and a cells encounter the opposite mating pheromones, they induce genes necessary for mating, arrest the cell cycle in G1, altering cell surface and…

RNaseRase Decontamination Solution is a surface decontamination solution that removes RNases on contact. Simply spray the surface to be decontaminated and wipe it off with nuclease-free water. Effectively removes RNase contamination from plastic and glass surfaces without inhibiting following…

TAE is used to prepare agarose gels and as an electrophoresis running buffer for the separation of double-stranded DNA in agarose and polyacrylamide gels. 50x TAE Buffer is composed of 2 M Tris-Acetate, 0.05 M EDTA, pH 8.3. For agarose gel electrophoresis, 50x TAE Buffer should be diluted to a…

Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL).

When yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes,…

Component of Viral RNA Purification Kit Up to 100 µg of total RNA binding capacity Optimized for COVID-19 viral RNA extraction from respiratory specimens Supplied with collection tubes Manufactured in FDA registered, ISO certified facility DNase/RNase/Proteinase free Sterile  

RNase A is endonuclease-free and has been rigorously tested for RNA digestion activity in plasmid purification procedures.

Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS, urea, and guanidine salts. It can be inactivated by phenyl methane sulfonyl fluoride…

Isopropyl-β-D-thiogalactopyranoside (IPTG).

The pre-filled format of Viral RNA extraction kit provides an easy and reliable method for simultaneously extracting total viral RNA from 96 samples in 25 minutes. Fully validated and automated on Allsheng auto pure 96 and Kingfisher plex, it can be adapted to major automation platforms such as…

Useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.