* Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins * Used in the isolation of RNA * Solubilizes insoluble or denatured proteins such as inclusion bodies * Can be used for the recovery of periplasmic proteins

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured…