1X concentration contains: 50mM Tris-HCl (pH 6.8) 2% SDS 10% Glycerol 1% β Mercaptoethanol 12.5mM EDTA 0.02% Bromophenol Blue

Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. Technical Information: 10% Horse serum, 0.02% sodium azide in PBS