Rockland Immunochemicals Inc Buffer Solutions
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Blocking Buffer
Rockland Immunochemicals IncBlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX (Licor), Cy2™,…
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TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
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1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED, 1L, Liquid …
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1.0 mM EDTA at a pH of 7.8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.
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Competent Cell Buffer (60 mM Calcium Chloride, 15% (v/v) Glycerol, 10 mM Tris…
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
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Blocking Buffer for Fluorescent Western Blotting, 500mL, Liquid (sterile…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…
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NORMAL RAT SERUM (NRS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Rat Serum (NRS) is a useful reagent for blocking Western Blots and ELISA wells.
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10X TBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A…
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TMB MEMBRANE PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB…
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10X PBS MaxTag™ Histo for IHC, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals Inc10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.2 M Potassium…
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UltraPure Sterile Water (4 x 1 liter), 4L, Liquid (sterile filtered)
Rockland Immunochemicals IncSuitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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FREUND'S COMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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1.0 M Tris HCl pH 8.5, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 8.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade (WFI) water. This product…
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BLOTTO A PRE-MIXED, 500mL, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…
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PolyMount Mounting Media MaxTag™ Histo for IHC, 15mL, Liquid
Rockland Immunochemicals IncThis component is ready-to-use and requires no additional preparation.
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1X PBS Buffer with 1% Casein
Rockland Immunochemicals IncThis product is a ready-to-use 1X solution and no further dilution is required.
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10X RIPA Lysis Buffer
Rockland Immunochemicals IncThis product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and…
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10X Tris-Phosphate-EDTA (TPE) (0.9 M Tris-Phosphate 0.01 M EDTA pH 8.0), 1L,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Phosphate EDTA (TPE) consists of 0.9 M Tris-Phosphate, 0.02 M EDTA at a pH of 8.0. Meticulously prepared using ultra…
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1.0 M Tris HCl pH 7.6, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…
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1X TBS Buffer with 1% Casei
Rockland Immunochemicals IncThis product is a ready-to-use 1X solution and no further dilution is required.
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20% (w/v) Sodium Dodecyl Sulfate (SDS)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 20% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly…
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TMB MEMBRANE PEROXIDASE SUBSTRATE. READY-TO-USE, 1L, Liquid - clear
Rockland Immunochemicals IncTMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB…
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1.0 M Magnesium Chloride DEPC TREATED, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 1.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains 1.0 M Magnesium Chloride and was meticulously prepared using ultra pure reagents dissolved in highly…
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Tris-Glycine
Rockland Immunochemicals IncTris-Glycine running buffer is suitable for laboratory involved in protein biochemistry. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X…
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10X BBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X BBS Fish Gel Concentrate consists of 1.0M boric acid, 1.5M sodium chloride and Fish Gelatin at pH 8.0. A proprietary…
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5.0 M Potassium Acetate (untitrated) DEPC TREATED
Rockland Immunochemicals IncThis product is a 5.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 5.0 M Potassium Acetate. Meticulously prepared using ultra pure reagents dissolved in highly…
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UniGlow™ Liquid
Rockland Immunochemicals IncUniGlow™ is ready-to-use and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent. Equilibrate to room temperature before use and aliquot into a…
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10X Tris-Borate-EDTA (TBE) (0.9 M Tris-Borate 0.01 M EDTA pH 8.3)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure reagents…
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TMB PRESTAINED RED ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB Prestained Red ELISA Peroxidase Substrate produces a soluble blue end product of ELISA, read at 370nm or 655nm.
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10X PBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X PBS Fish Gel Concentrate consists of 0.2M potassium phosphate, 1.5M sodium chloride and Fish Gelatin at pH 7.2. A…
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TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.
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UltraPure Sterile Water, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncUltra Sterile Water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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ELISA Microwell Coating Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncELISA Microwell Coating Stabilizer is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to preforming ELISA. After the addition of blocking agent, wash the contents of ELISA microwells and add a sufficient volume of ELISA Microwell Coating Stabilizer to each well of the…