Rockland Immunochemicals Inc Buffer Solutions
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10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium…
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FREUND'S COMPLETE ADJUVANT
Rockland Immunochemicals IncFreund’s adjuvant may be used to form water-in-oil emulsions with antigens for the injection of immunogens for antibody production. Proper emulsion formation is essential and should be tested by observing a drop of the emulsion on the surface of water which should 'bead and not disperse. Antigens…
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10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished…
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DAB MEMBRANE PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncDAB Membrane Peroxidase Substrate (50x) is designed to be diluted with part number DAB-B50
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ALP Conjugate Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncDilute your primary or secondary alkaline phosphatase conjugated antibody or other protein or compound with ALP Conjugate Stabilizer to the desired 1X working concentration determined for your conjugate. ALP Conjugate Stabilizer may be used to dilute conjugates when determining titer. In one step…
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Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml), 1Each,…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye™800,…
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Blocking Buffer
Rockland Immunochemicals IncBlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX (Licor), Cy2™,…
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Normal Saline, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncSaline solutions are available in various formulations for different purposes. Salines are also used in cell biology, molecular biology, and biochemistry experiments where ever normal salines are needed.
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1X RIPA Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncRIPA Lysis Buffer is ready-to-use as a working 1X solution and requires no further dilution. RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…
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1X TBS pH 7.8 (0.1M Tris HCl 0.15M Sodium Chloride)
Rockland Immunochemicals IncThis product is a 1X working solution. 1X Tris Buffered Saline consists of 0.1M Tris HCl and 0.15 M Sodium Chloride, pH 7.8, meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI).
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10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20), 1L,…
Rockland Immunochemicals IncTBST is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. May be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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2X SDS-PAGE Sample Buffer, 1.5mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. The pH of…
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Molecular Biology Grade UltraPure Water
Rockland Immunochemicals IncMolecular Biology Grade Ultrapure water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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ProXtract B™ Extraction Buffer
Rockland Immunochemicals IncProXtract B™ Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol.
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GET Buffer
Rockland Immunochemicals IncThis product is a 1X concentrated stock solution. No dilution is required. This buffer consists of 50 mM Glucose, 10 mM EDTA, and 25 mM TRIS Cl at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade deionized water.
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HRP Conjugate Stabilizer (Azide and Mercury Free), 100mL, Liquid (sterile…
Rockland Immunochemicals IncThis product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline, and proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A…
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NORMAL HORSE SERUM (NHS), 10mL, Lyophilized
Rockland Immunochemicals IncHorse serum is suitable for blocking, western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
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NORMAL RABBIT SERUM (NRS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Rabbit Serum (NRS) is a useful reagent for blocking Western Blots and ELISA wells.
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ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncThis product is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stabilizer to each well of the microplate. …
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BLOTTO A PRE-MIXED, 500mL, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…
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10X PBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X PBS Fish Gel Concentrate consists of 0.2M potassium phosphate, 1.5M sodium chloride and Fish Gelatin at pH 7.2. A…
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10% (w/v) Sodium Dodecyl Sulfate (SDS), 100mL, Liquid (sterile filtered)
Rockland Immunochemicals Inc10% SDS is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly polished…
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20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC…
Rockland Immunochemicals Inc20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH…
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10X Tris-Borate-EDTA (TBE) (0.9 M Tris-Borate 0.01 M EDTA pH 8.3)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure reagents…
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TMB PRESTAINED RED ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB Prestained Red ELISA Peroxidase Substrate produces a soluble blue end product of ELISA, read at 370nm or 655nm.
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10X TBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A…
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BCIP/TNBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to very…
Rockland Immunochemicals IncBCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for WB, IHC, and in situ hybridization.
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5.0 M Ammonium Acetate (untitrated) DEPC TREATED, 100mL, Liquid (sterile…
Rockland Immunochemicals Inc5.0 Ammonium Acetate is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 5.0 M Ammonium Acetate. Meticulously prepared using ultra pure reagents dissolved in highly…
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Blocking Buffer for Fluorescent Western Blotting, 500mL, Liquid (sterile…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…
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Cell Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThe buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.
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Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x…
Rockland Immunochemicals IncOptimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary…