PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work.

MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. Concentrations higher than 20 mM should not be used with mammalian cell cultures.

MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable,…

MES, Sodium Salt (2-(N-Morpholino)ethanesulfonic acid sodium salt) is a zwitterionic N-substituted aminosulfonic acid known as a Good's buffer, active in the pH range 6-8 with a pKa of 6.10 at 25° C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it…

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to…

MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature chemically and…

Used as a buffer in biological research. HEPPSO is an efficient separator in protein resolution by IEF. HEPPSO is a Zwitterionic hydrogen buffer.