Western Blotting and ELISA Chemicals
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Flash Western Chemiluminescence Kit – Rabbit
Azure BiosystemsKit includes all reagents necessary for performing ten (10) chemiluminescent Western blots using the Flash Western protocol – includes LF PVDF membranes, Azure Blocking Buffer, Flash anti-rabbit HRP Conjugate, Flash Wash Buffer and Radiance ECL substrate
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Well-Coated™ Glutathione Plates
G-BiosciencesWell-Coated™ Glutathione plates are designed to specifically bind GST (Glutathione S-Transferase) tagged proteins and peptides. The plates have immobilized glutathione and can be used for the isolation of GST-tagged proteins direct from bacterial lysates and then used for subsequent ELISA…
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Western ReProbe™
G-BiosciencesWestern ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots. Western ReProbe™…
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TMB Stain Kit
Bio Basic Inc.TMB Stain Kit: 3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualizing reagent used in enzyme-linked immunosorbent assays (ELISA). In solution, TMB forms a blue product when allowed to react…
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FLASHBlot™ Transfer Buffers
AdvanstaEnhanced protein transfer for improved sensitivity Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more…
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BLOK™ BSA
G-BiosciencesProtein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system. Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum Albumin) …
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Flash Western Demo Kit – Rabbit
Azure BiosystemsKit for testing the chemiluminescent Flash Western blot protocol – includes all reagents necessary for testing the Flash Western protocol including control lysate, molecular weight marker, LF PVDF membrane, control primary antibody, sample buffer, transfer buffer, blocking buffer, HRP conjugate,…
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AzureSpectra Labeling Kit – for Western Blot – 490
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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Quenching Sheets
Azure Biosystems10 background quenching sheets for use with fluorescent or chemiluminescent samples (9x12.5 cm)
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Low Fluorescence Western Membrane (LF PVDF) 0.45um (9x7)
Azure BiosystemsPre-cut LF PVDF membrane (9x7 cm mini vertical gels); 10 sheets
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femtoCHROMO™-HRP Kit
G-Biosciencesfemto CHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein band s with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…
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AzureSpectra Labeling Kit – for Western Blot – 650
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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AzureSpectra Labeling Kit – for Western Blot – 550
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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Azure Transfer Buffer
Azure BiosystemsTransfer buffer (50x concentrated solution) sufficient for 50-100 blots; 500 ml
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Ultra Thick Blotting Paper, Grade 320
Ahlstrom-MunksjoThis super thick grade is one of the thickest papers on the market, and offers extremely high flow. It is recommended both for electrophoresis of large molecules and as a blotter to complete the blotting sandwich in gel transfer applications. This blotting paper provides a smooth and uniform…
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10X Fish Gelatin Blocking Agent
Biotium10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum…
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Western Blot Blocker
bioWORLDWestern Blot Blocker (Thomas No. C994D14 and C99 D15) For those that prefer animal protein as the blocking agent for your immuno-detection analysis. Modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking…
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Azure Protein-Free Blot Blocking Buffer
Azure BiosystemsProtein-free blocking solution for Western blot, 5x concentrate; 200 ml
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Swift Western Diluent
G-BiosciencesThe Swift™ Western Diluent is a unique solution that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes. Swift™ Western Diluent generates comparable result to traditional Western blotting procedures and other commercial…
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picoLUCENT™ PLUS-HRP Kit
G-BiosciencespicoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-HRP reagents are…
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Blot Development Folder
Azure BiosystemsTransparent plastic sheets to hold Western blots for imaging – alternative to plastic wraps; 100 pcs
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PBST buffer 10x Strength Solution
Bio Basic Inc.The Bio Basic Inc. PBST buffer 10x strength solution is suitable for laboratory and research use.
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Azure Fluorescent Blot Blocking Buffer
Azure BiosystemsBlocking solution for fluorescent Western blot, 1x; 500 ml
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AzurePonceau Stain
Azure BiosystemsVisible reversible total protein stain for proteins transferred to membrane – sufficient for staining 50 blots 9x7 cm; 500 ml
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BLOK™ BLOTTO (5% modified milk protein solution)
G-BiosciencesConvenient ready-to-use blocking solution for Western blots, dot Blots & ELISA Features 5% novel modified milk protein solution Use for Westerns, ELISA, and dot Blot blocking Blocking in 10-15 minutes Ready-to-use
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Low Fluorescence Western Membrane (LF PVDF) 0.45 um (13x18)
Azure BiosystemsPre-cut LF PVDF membrane (13x18 cm vertical gels); 10 sheets
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Well-Coated™ Sulfhydryl Binding Plates
G-BiosciencesWell-Coated ™ Sulfhydryl Binding plates are designed to specifically bind free sulfhydryls of peptides, proteins and other molecules. The Well-Coated ™ Sulfhydryl Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other…
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picoLUCENT™ PLUS AP Kit
G-BiosciencespicoLUCENT™ PLUS-AP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-AP is also…
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femtoCHROMO™-AP Kit
G-Biosciencesfemto CHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…
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Well-Coated™ Protein A Plates
G-BiosciencesWell-Coated ™ Protein A plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site. The immunoglobulin orientation on the Well-Coated ™…
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Well-Coated™ Protein L Plates
G-BiosciencesWell-Coated ™ Protein L plates are designed to bind the kappa light chains of immunoglobulins without interfering with the antigen binding site. The Well-Coated ™ Protein L plates offer greater range of immunoglobulin classes and subclasses compared to Protein A , Protein G and…