This assay product utilizes proprietary human cells engineered to provide constitutive, highlevel expression of the Human Pregnane X Receptor (NR1I2), a ligand-dependent transcription factor commonly referred to as PXR. PXR is also known as the Steroid and Xenobiotic sensing nuclear receptor (SXR).…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 2 (NR3A2), a ligand-dependent transcription factor commonly referred to as ERβ. INDIGO’s reporter cells include the…

4-(hydroxymercuri)Benzoic Acid(PHMB) is used to inhibit cysteine-protease and acts as a reversible thiol-specific blocker. While blocking the acylated ghrelin degradation, it allows the AcetylCholine Esterase AChE™ used in SPI-Bio EIA kits to work normally, in contrast to serine-protease inhibitors.

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human thyroid hormone receptor beta (NR1A2), a ligand-dependent transcription factor commonly referred to as TRβ. INDIGO’s reporter cells include the…

Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as ‘Host Cell Proteins (HCP)', are generated and need to be removed. This kit…

Water used for the preparation of EIA reagents and buffers.

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, alpha (NR1B1), a ligand-dependent transcription factor commonly referred to as RARA or RARα. INDIGO’s reporter cells…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoid X receptor beta (NR2B2), a ligand-dependent transcription factor commonly referred to as RXRB or RXRβ. INDIGO’s reporter cells include…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, beta (NR1B2), a ligand-dependent transcription factor commonly referred to as RARB or RARβ. INDIGO’s reporter cells…

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor beta (NR1H2), a ligand-dependent transcription factor commonly referred to as LXRβ. INDIGO’s reporter cells include the…

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO’s reporter cells…

For the measurement of acylated ghrelin.

CGRP is a 37 amino acid peptide synthesized in the central and peripheral nervous system from a calcitonin/CGRP gene complex. Two isoforms have been described which differ by three amino acids and display similar biological activities: CGRP-α, which is produced by alternative splicing of a…

BHT is a phenolic antioxidant and free radical scavenger. It has been shown to inhibit lipid peroxidation.

INDIGO’s panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. INDIGO’s TR reporter cells include the…

Features Allows insulin measurement even in hemolyzed samples Contains insulinase inhibitors to protect sample integrity during collection The main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor gamma (NR1B3), a ligand-dependent transcription factor commonly referred to as RARG or RARγ. INDIGO’s reporter cells…

Indomethacin is a COX inhibitor that prevents ex vivo TXB2 formation and other PGs that can not be prevented using anticoagulant alone. The samples have to be transferred in indomethacin sampling tubes as soon as possible after blood collection.

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO’s reporter cells include the luciferase reporter…

This nuclear receptor assay system utilizes a proprietary rodent cell line that is further engineered to express the mouse/rat peroxisome proliferator-activated receptor gamma (NR1C3, PPARG). Because the receptor’s ligand binding domain sequence is conserved between mouse and rat species, the…

Lyophilized fluorescent substrate.

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified human mineralocorticoid receptor (NR3C2), a ligand-dependent transcription factor commonly referred to as MR. INDIGO’s reporter cells…

This nuclear receptor assay system utilizes proprietary human mammalian cells engineered to provide high-level expression of a hybrid form of the human estrogen-related receptor alpha (NR3B1). The N-terminal DNA binding domains (DBD) of the native ERRα has been substituted with that of the…

This EIA is based on the competition between free adiponectin and coated adiponectin coated to the wells of a 96-well plate for a fixed quantity of HRP-labeled adiponectin antibody.

Obestatin is an amidated peptide of 23 amino-acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and…

INDIGO’s panel of ER assays utilizes non-human mammalian cells engineered to express human estrogen receptors alpha (ER1; NR3A1) and beta (ER2; NR3A2), ligand-dependent transcription factors commonly referred to as ERα and ERβ. INDIGO’s ER reporter cells include the…

Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a ubiquitously expressed, basic leucine zipper transcription factor. It regulates the expression of a variety of genes,encoding proteins that play critical roles in cyto-protection, as well as the detoxification and clearance of harmful…

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor that is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that control energy homeostasis. Ghrelin is present in the peripheral circulation in two…

For quantification of 20-hydroxyecdysone in a variety of biological samples.

Atriopeptin is a 28 amino acid peptide synthesized primarily in cardiac atria. This peptide hormone acts in opposition to angiotensin II in regulating renal, hemodynamic, and endocrine function. Atriopeptin is released in response to the increased pressure and mechanical stretch of the right atrium…

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human farnesoid X receptor (NR1H4), a ligand-dependent transcription factor commonly referred to as FXR. INDIGO’s reporter cells include the…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the mouse peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or mPPARα.…