Cayman’s Glycerol Cell-Based Assay provides a convenient tool for studying triglycerides/fatty acid cycling and its regulation in adipocytes or hepatocytes. This kit will allow investigators to screen compounds involved in lipid storage and metabolism. Chloroquine is included in the kit as a…

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including…

Early screening of a drug candidate’s potential to induce or inhibit CYP1A1/2 is a critical step in the drug development process. Cayman's CYP1A1/2 Induction Reporter Assay Kit is a novel method to assess CYP1A2 induction. Our Reverse Transfection Reporter Assays have overcome many of the…

Angiotensin II is a primary reactive vasoconstrictor, the main stimulus for aldosterone release, and one of the causative factors of chronic hypertension. The active angiotensin II octapeptide is released via a tightly controlled series of prohormones and proteases. Normal human plasma angiotensin…

Cayman’s PAF-AH Inhibitor Screening Assay uses 2-thio PAF as a substrate for PAF-AH. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using DTNB also known as Ellman's reagent. Cayman's PAF-AH Inhibitor Screening assay includes…

Mitochondrial membrane potential, Δψm, is an important parameter of mitochondrial function that is used as an indicator of cell health. JC-1 is a lipophilic, cationic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential…

Caspase-3 plays a central role in the execution of apoptosis. Cayman’s Caspase-3 Fluorescence Assay employs a specific caspase-3 substrate, N-Ac-DEVD-N'-MC-R110, which upon cleavage by active caspase-3, generates a highly fluorescent product that can be measured using excitation and…

When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman’s…

This EIA kit specifically measures the acylated form of ghrelin.

Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis. In this kit,…

Cayman’s 8-Isoprostane Affinity Column provides a specific and convenient means for the purification of 8-isoprostane (8-iso PGF2α) from urine and plasma samples. Each column contains 0.5 ml of affinity sorbent (mouse anti-8-isoprostane monoclonal antibody covalently bound to Sepharose 4).

Lyophilized fluorescent substrate.

Glutathione (GSH) is involved in maintenance of protein sulfhydryl reduction status. PSSG may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. Cayman’s PSSG provides a convenient method for the direct visualization…

Cayman’s Cell Cycle Phase Determination provides an easy to use tool for studying the induction and inhibition of cell cycle progression in any cell suspension sample. The assay involves the fixation and permeabilization of the cells of interest, making possible the staining of DNA within…

Lyophilized fluorescent substrate.

CGRP is a 37 amino acid peptide synthesized in the central and peripheral nervous system from a calcitonin/CGRP gene complex. Two isoforms have been described which differ by three amino acids and display similar biological activities: CGRP-α, which is produced by alternative splicing of a…

Cayman's MC3R Reporter Assay consists of a 96-well plate coated with a transfection complex containing DNA constructs for MC3R and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (MC3R Reverse Transfection Strip Plate). Cells grown on the transfection complex…

Purified MIS for ELISAs.

Cayman's OX2R Reporter Assay Kit consists of a 96-well plate coated with both OX2R and Secreted Alkaline Phosphatase (SEAP) reporter constructs (OX2R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express OX2R at the cell surface and the recombinant G-protein…

Cayman's Multi-Drug Resistance Assay Kit provides a convenient tool for studying the modulation of cellular multi-drug resistance (MDR) machinery. The kit employs calcein AM, a cell-permeable nonfluorescent dye, which is cleaved intracellularly to yield the impermeant fluorescent molecule,…

The assay utilizes plates coated with a monoclonal capture antibody specific for the human leptin receptor and a HRP-conjugated monoclonal antibody for detection.

Follicle-Stimulating hormone (FSH) is a glycoprotein produced by the pituitary gland and consists of two subunits with an approximate total molecular mass of 35 kDa. In males, FSH acts on the Sertoli cells of the testis to stimulate the synthesis of androgen-binding protein, which is indirectly…

AcSDKP is a tetrapeptide growth regulatory hormone which inhibits the proliferation of hematopoietic stem cells. The dipeptidase angiotensin converting enzyme (ACE) actively metabolizes circulating AcSDKP, giving it a brief plasma half-life of 4 to 5 minutes. ACE inhibition is a major therapeutic…

Insulin is a polypeptide hormone with a molecular weight of 6 kDa, composed of two peptides chains, A and B, cross-linked by two disulfide bonds and synthesized by the b cells of the islets of Langherans of the pancreas. Insulin influences many metabolic functions of the body. Its best known action…

FABP4 is a 15 kDa member of the intracellular FABP family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids). FABP4 is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function…

Application: ELISA and WB

A leptin standard set.

Cayman’s Adipogenesis Assay provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model, using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O…

The NOS Activity Assay measures NOS activity by monitoring the conversion of radiolabeled arginine to citrulline. This assay is simple, sensitive, and specific for NOS activity and can be used with both crude and purified enzyme preparations. The kit includes sufficient materials and reagents for…

The 20S proteasome is the proteolytic core of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases.…

Leptin is a 16 kDa protein hormone encoded by the obese (ob) gene with important effects in metabolism and regulation of body weight. Leptin has dual actions, decreasing appetite and increasing energy consumption. The primary effect of leptin appears to be mediated by leptin receptors expressed…

FFAR4/GPR120 is a G protein-coupled receptor for long chain fatty acids such as DHA and EPA ω-3 fatty acids. It is involved in the regulation of glucose uptake in adipocytes and mediates anti-inflammatory activity in macrophages. Cayman’s FFAR4 (GPR120) Reporter Assay Kit consists of a…