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IBI Scientific

Genomic DNA Kits (Tissue)

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Extract Genomic DNA from Various Animal Tissues

The Genomic DNA MINI (Tissue) Kits are specially designed for purification of total DNA (including genomic mitochondrial and viral DNA) from a variety of animal tissues and cells. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis step. This method uses Proteinase K and a chaotropic salt to lyse cells and degrade proteins. The DNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. This entire procedure can be completed in under 60 minutes without phenol extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50mg and the purified DNA (with approximately 20-30kb) is suitable for PCR or other enzymatic reactions.

Sample Size:up to 50mg of tissue
Format:Spin Column
Operation:Centrifuge / Vacuum Manifold
Binding Capacity:50µg
Expextant Yield:up to 50µg
Operation Time:60 minutes or less
Elution Volume:50-200µl
Applications:PCR, AFLP/PADP, RFLP, Southern Blotting, Real-Time PCR

Kit Components

GD Columns4 MINI50 MINI300 MINI
2mL Collection Tubes8100600
GT Buffer1.5mL30mL75mL
GB Buffer2mL30mL75mL
W1 Buffer2mL45mL130mL
Wash Buffer (add Ethanol)1mL (4mL)25mL (100mL)50mL (200mL)
Elution Buffer1mL30mL75mL
Proteinase K (add ddH2O)1mg (0.1mL)11mg (1.1mL)65mg (6.5mL)

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Thomas No.
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Mini Genomic DNA Kit (Tissue)-4 Prep
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Thomas No.
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Mini Genomic DNA Kit (Tissue)-50 Prep
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Mini Genomic DNA Kit (Tissue)-300 Prep
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