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IBI Scientific

Genomic DNA Kits (Plant)

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Extract Genomic DNA from Plant and Fungal Tissues

The Genomic DNA MINI and MAXI (Plant) Kits provide a fast and economical method to isolate total DNA (genomic DNA, mitochondrial and chloroplast) from plant tissue and cells. Samples are disrupted both by grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix of the spin column. The contaminants are washed with an ethanol based wash buffer. The Genomic DNA is then eluted by a low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. This entire procedure can be completed in 30 minutes or less and the purified genomic DNA is ready for PCR, Real-Time PCR, Southern Blotting, or RFLP.

Sample Size:MINI - up to 100mg fresh plant tissue / 25mg of dry plant tissue
MAXI - 1gm of fresh plant tissue / 250mg of dry plant tissue
Format:Spin Column
Operation:Centrifuge / Vacuum Manifold
Binding Capacity:MINI - 50µg / MAXI - 500µg
Expextant Yield:MINI - up to 50µg of DNA /
MAXI - up to 140µg of DNA
Operation Time:MINI - 60 minutes or less /
MAXI - 60 minutes

Kit Components

2mL Collection Tubes8200---
Filter Column410010252
GP1 Buffer2mL50mL50mL125mL10mL
GPX1 Buffer2mL50mL50mL125mL10mL

GP2 Buffer

GP3 Buffer (add Isopropanol)1.5mL (3mL)30mL (60mL)30mL (60mL)70mL (140mL)8mL (16mL)
W1 Buffer2mL45mL45mL130mL10mL
Wash Buffer (add Ethanol)1mL (4mL)25mL (100mL)25mL (50mL)50mL (200mL)5mL (20mL)
Elution Buffer1mL30mL30mL60mL6mL
RNase A (10mg/mL)25µL550µL550µL(2) 650µL100µL

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Product Detail
Thomas No.
Mfr. No.
Also known as: DP-7230
Mini Genomic DNA Sample Kit (Plant)-4 Prep
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Thomas No.
Mfr. No.
Also known as: DP-7231
Mini Genomic DNA Kit (Plant)-100 Preps
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