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IBI Scientific

96-Well PCR Clean-Up Kits

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High-Throughput PCR Clean-Up

The 96-Well PCR Clean-Up/DNA Extraction Kits provide a highthroughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR and sequencing reactions.

Sample Size:

up to 50µl of PCR product
up to 50mg of agarose slice

Format:96-Well Plates
Operation:Centrifuge/Vacuum manifold
Binding Capacity:10µg per well
DNA Size:50bp - 10kb
Recovery:80-90% for Gel Extraction
90-95% for PCR Clean-Up
Operation Time:30 minutes for PCR Clean-Up
40 minutes for Gel Extraction

Kit Components

DNA Binding Plates4x9610x96
0.35mL Collection Plates410
Adhesive Film820
Binding Buffer120mL120mL
Wash Buffer (add Ethanol)50mL (200mL)(2) 50mL
((2) 200mL)
Elution Buffer30mL60mL

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Product Detail
Thomas No.
Mfr. No.
Also known as: DP-7040
4x96-Well PCR Clean-Up Kit-4 Plates
list price/quantitytotal
Thomas No.
Mfr. No.
Also known as: DP-7050
10x96-Well PCR Clean-Up Kit-10Plates
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