ACTaq™ Blue / Red Mastermix is complete “ready-to-go” 2X reaction mix which only requires consumer researcher to add water, template and primers. ACTaq™ Blue / Red Mastermix differs from ACTaq™ Mastermix such that it contains blue / red dye added to the enzyme used for traccking adn loading dye purposes, and therefore no loading buffer is required. Taq ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75ºC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.
|Storage Condition:||-20ºC. Stable for 12 months in constant freezer temperature|
|Unit Definition:||One unit incorporates 10nmol dNTP into acid-insoluble material in 30|
minutes at 72ºC.
|Product Compostition:||2.5 Units of ACTaq™ Blue / Red Taq DNA Polymerase, 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl, 16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.||Quality Control:||ACTaq™ Blue / Red Taq DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the|
enzyme is evaluated by SDS-PAGE at >95% purity.