Mueller Kauffman Tetrathionate Broth Base is used for improved enrichment and selective isolation of Salmonellae. Kauffman modified the formula to include bile and brilliant green as selective agents to suppress bacteria such as Proteus species. It is also a recommended selective broth for isolating Salmonella from animal feces and sewage-polluted water. Selectivity is conferred by tetrathionate (from the reaction of thiosulphate and iodine). This media also conforms to ISO specifications. Organisms other than Salmonellae, such as Morganella morganii and some Enterobacteriaceae may grow in the medium. Therefore, confirmatory tests should be carried out on all presumptive Salmonella colonies that are recovered.
Application: For Improved Enrichment and Isolation of Salmonellae