IL-1beta human, recombinant, expressed in HEK 293 cells, >=95% (SDS-PAGE)

Synonyms: Catabolin; Endogenous pyrogen (EP); Leukocyte endogenous mediator (LEM); Lymphocyte-activating factor (LAF); Mononuclear cell factor (MCF)

Purity: >=95% (SDS-PAGE)

Storage: -70C

UNSPSC Code: 12352200

General description: Interleukin-1+¦ (IL1B) is produced by activated macrophages. It is synthesized as pro-IL-1+¦ which is changed to active form IL-1+¦ with the help of pro-inflammatory protease caspase-1. IL-1+¦ belongs to the IL-1 gene family. It is a proinflammatory cytokine. The gene is mapped to human chromosome 2q14. In comparison with the Escherichia coli expressed protein, IL-1+¦ is 50% more potent in promoting human CD4+ T cell differentiation into Th17 (T helper) cells. This cytokine is produced in a serum free, chemically defined media.

Application: . IL-1+¦ (IL1B) has been used to check the expression of NGAL (neutrophil gelatinase-associated lipocalin) in epithelial cells after stimulation with proinflammatory cytokines. . It has been used to check anti-inflammatory activities of N-Acetylglucosamine in human chondrocytes. . It has been used to create inflammatory endothelium in human coronary artery endothelial cells.

Biochem/physiol Actions: IL-1+¦ (IL1B) stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation and fibroblast growth factor activity. IL-1+¦ proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1+¦ is present in chronic inflamed tissues and also in tissues going for fibrogenesis. It brings neutrophil and macrophage to the site of infection. Mutations in IL-1+¦ gene are linked with renal manifestations and renal sequelae in Henoch-Sch+¦nlein purpura. It is also associated with destruction of +¦-cell in type 1 diabetes.

Physical form: Lyophilized from a PBS solution.

Reconstitution: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Preparation Note: Centrifuge the vial prior to opening.