HGF human, recombinant, expressed in HEK 293 cells, >=95% (SDS-PAGE)

Synonyms: F-TCF; HPTA; Hepatocyte growth factor; Hepatopoietin-A (HPTA); Scatter factor (SF)

Purity: >=95% (SDS-PAGE)

Storage: -70C

UNSPSC Code: 12352200

General description: HGF (hepatocyte growth factor) is a multifunctional heterodimeric mitogen, with a relative molecular mass (Mr) of 82,000, consisting of a large +¦-subunit of Mr 69,000 and a small +¦ -subunit of Mr 34,000. The pre-pro precursor protein is composed of 728 residues. HGF is produced by mesenchymal cells.

Biochem/physiol Actions: HGF (hepatocyte growth factor) functions as a ligand for tyrosine kinase receptor Met. Through interaction with Met receptor, it modulates mitogenesis, motility, and morphogenesis in a cell-specific manner. It is implicated in the growth and scattering of specific cell types, EMT (epithelial mesenchymal transition), formation of lumens and tubules, and angiogenesis. HGF-Met is also involved in neural induction, hepatic regeneration, wound healing, and normal fetal development. In primary culture, HGF acts as a strong mitogen for mature parenchymal hepatocytes, and is thought to be a hepatotrophic factor which induces liver regeneration post-partial hepatectomy and liver injury. In HER2-positive patients with metastatic gastric cancer, the serum level of this protein is linked with the outcome and resistance to trastuzumab therapy.

Physical form: Lyophilized in solution of 10 mM Tris-HCl, pH 7.4, with 1 M NaCl.

Reconstitution: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Preparation Note: Centrifuge the vial prior to opening.