BMP-4 human, recombinant, expressed in HEK 293 cells, >=95% (SDS-PAGE)

Synonyms: BMP-2B; Bone morphogenetic protein 2B; Bone morphogenetic protein 4

Purity: >=95% (SDS-PAGE)

Storage: -20C

UNSPSC Code: 12352200

General description: BMP4 (bone morphogenetic protein 4), a member of the highly conserved BMP family, is a growth factor and a pro-inflammatory and pro-atherogenic cytokine. BMPs are part of the TGF+¦ (tumor growth factor) superfamily of proteins. BMP4 gene is localized to human chromosome 14q22-q23.

Biochem/physiol Actions: BMP4 (bone morphogenetic protein 4) is involved in modulating cell development in a wide variety of cells. It promotes the proliferation and migration of hepatocellular carcinoma cells, and the multiplication of ALK3 (activin-like kinase)-positive cells in skeletal muscle. This protein prevents the proliferation of lung fibroblasts and induces their differentiation into myocytes. In Sertoli cells, it promotes DNA synthesis and hence, the proliferation of Sertoli cells through Smad1/5 (small mothers against decapentaplegic) and ID2/3 (DNA binding protein inhibitor) pathway, and aberration in BMP4 expression results in azoospermia. In human males, the serum levels of BMP4 is linked with coronary artery disease (CAD) severity. BMP4 is crucial for maintaining the self-renewal capacity of ES (embryonic stem) cells, and achieves so by inhibiting extracellular receptor kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathways.

Physical form: Lyophilized from a PBS solution.

Reconstitution: Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin.

Preparation Note: Centrifuge the vial prior to opening.