Escherichia coli is commonly used as a model organism for bacteria in general. Because of its ubiquity, E. coli is frequently studied in microbiology and is the current “workhorse” in molecular biology. E. coli plays an important role in modern biological engineering. Researchers can alter the bacteria to serve as “factories” to synthesize DNA and/or proteins, which can then be produced in large quantities using the industrial fermentation processes. One of the first useful applications of recombinant DNA technology was the manipulation of E. coli to produce human insulin for patients with diabetes. E. coli grow rapidly in NZ Amine A Broth (1) as it provides necessary metabolites for the cell, which would otherwise have to be synthesized by the cell (2).
Casein acid hydrolysate and NZ Amine A provide necessary nutrients and cofactors required for the growth of E. coli. Sodium chloride helps to maintain the osmotic balance whereas magnesium ions serves as immediate activator required for a number of enzymatic reactions, including DNA replication of the cell.
Directions: Suspend 18 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.