Mycological Peptone is a mixture of animal and plant peptones required for obtaining luxuriant growth of fungi-yeasts and moulds. It is equivalent to Peptone M. It is recommended in culture media for isolation and diagnosis of pathogenic and non pathogenic fungi and identification of saprophytic and dermatophytic fungi-yeasts and moulds. It is light yellow in color and does not mask the appearance of media containing dyes and indicators. When dissolved in distilled water it produces light yellow colored solution that remains clear even after autoclaving. It is also compatible with other media constituents and will not precipitate in media. 1.0% w/v solution of it, provides a broad spectrum of amino acids required for mycelial growth and sporulation.It is equivalent to Peptone M.
- Appearance: Light yellow to brownish yellow homogeneous free flowing powder characteristic but not putrescent
- Solubility: Freely soluble in distilled/purified water, insoluble in alcohol
- Clarity: 1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes
- Bacterial Count: <= 2000 CFU/gram by plate method, when incubated at 30-35ºC for not less than 3 days
- Yeast & mould Count: <= 100 CFU/gram by plate method, when incubated at 20-25ºC for not less than 5 days
- Test for pathogens: 1. Escherichia coli- Absent/gram of sample 2. Salmonella species- Absent/10 gram of sample 3. Pseudomonas aeruginosa- Absent/gram of sample 4. Staphylococcus aureus- Absent/gram of sample 5. Candida albicans- Absent/gram of sample 6. Clostridia- Absent/gram of sample
- Indole Test: Tryptophan content: Passes
- Cultural response: Cultural response observed after an incubation at 25-30°C for 48-72 hours by preparing Malt Extract Agar (M137) using Mycological Peptone as an ingredient