Kings Medium A is recommended for non-selective isolation, cultivation and pigment production of Pseudomonas species. It is particularly suited for the production of pyocyanin and pyorubin. Pigments and/ or their derivatives produced by Pseudomonas species play a role as siderophores in the iron uptake systems of bacteria, and hence, their production is markedly enhanced under conditions of iron deficiency. Pseudomonas aeruginosa produces blue-green pigment, pyocyanin.
Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis for Invitro Diagnostic Use
- Appearance: Cream to yellow homogeneous free flowing powder
- Gelling: Firm, comparable with 1.5% Agar gel
- Color and Clarity of prepared medium: Light yellow colored, clear to slightly opalescent gel forms in Petri plates
- Reaction: Reaction of 4.6% w/v aqueous solution (containing 1.0%v/v glycerol) at 25°C. pH : 7.3±0.1
- Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours