It can be used for the preparation of media for cultivation of following bacteria: Neisseria species : G C HiVeg Agar Base
(MV434), Thayer Martin HiVeg Medium Base (MV413) Yersinia species : Yersinia Selective HiVeg Agar Base (MV843).
Staphylococci and Streptococci : Columbia Blood Agar Base HiVeg (MV144) . It can also be used for cultivation of other
fastidious bacteria on large scale.
Principle And Interpretation
HiVeg Special Peptone is manufactured under controlled conditions from vegetable proteins. It is especially adapted for the
preparation of media for culturing fastidious bacteria and supports cultural characteristics comparable with Peptone special
(RM015).
Quality Control
Appearance
Light yellow to yellow, may have a slight green tinge ,Homogenous Free flowing powder, having Characteristic odour of protein,
derived from vegetable source.
Solubility
Freely soluble in distilled/ purified water, insoluble in alcohol.
Clarity
1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC ) for 15 minutes.
Reaction
Reaction of 2%w/v aqueous solution at 25ºC.
pH
5.50- 7.50
Microbial Load:
Total aerobic microbial count (cfu/gm)
By plate method when incubated at 30-35°C for not less than 3 days.
Bacterial Count : <= 2000 CFU/gram
Total Yeast and mould count (cfu/gm)
By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mould Count : <= 100 CFU/gram
Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa-
Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of
sample6. Clostridia- Negative in 10 gms of sample
Indole test
Tryptophan content: Passes
Cultural response
Cultural response observed after incubation at 35 - 37°C for 18-48 hours by preparing Columbia Blood Agar Base HiVeg
(MV144) using HiVeg Special Peptone as an ingredient.
Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.