Hiveg medium used for testing the sterility of biological products and for isolation of aerobic and anerobic organisms. If more than the upper one third layer acquires bluish-green color (absorbs oxygen), the dissolved oxygen can be removed by heating the medium in free flowing steam for 5-10 minutes or in a water bath until the green color disappears, and the prepared medium should be stored in the dark till use. The uninoculated medium shows bluish green color at the top indicating presence of oxygen in that part. Organisms that ferment dextrose and lower the pH to critical levels may not survive in this medium after growth has taken place.
- Appearance of Powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder
- Color and Clarity: Yellow colored, clear to very slightly opalescent fluid with upper 10% or less medium bluish green on standing
- Reaction of 4.05% w/v of MV019 or 3.85% w/v of MV195 aqueous solution is pH 7.2 ± 0.2 at 25°C
- Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours