The medium is a highly nutritious medium which can support luxuriant growth of wide variety of microorganisms including bacteria, yeasts and moulds and is often used for isolation of pathogens from clinical specimens especially blood. Para amino benzoic acid is an active inhibitor of the bacteriostasis produced by the sulfonamide drugs; also it serves as an accessory growth factor for several species of bacteria. Therefore para amino benzoic acid incorporated in the medium helps to neutralize the effect of antimicrobials present in the blood of patients under sulphonamide therapy making isolation of organisms from blood easier. Agar in the medium reduces the oxygen tension and favors growth of facultative and obligatory anaerobic microorganisms.
Application: For Used For Culturing Blood From Patients Under Sulphonamide Therapy, the Addition of Agar Improves Growth of Anaerobes
- Appearance of Powder: yellow coloured, homogeneous, free flowing powder
- Colour and clarity: light amber coloured, clear to very slightly opalescent solution without any precipitate
- Reaction of 3.7% w/v of MV212 or 3.8% w/v of MV213 aqueous solution is pH 7.4 ± 0.2 at 25°C
- Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours, in presence of added 0.5 grams sulphadiazine per litre