The medium is highly nutritious thus supports luxuriant growth of Bordetella species and can also be used for mass cultivation of B. pertussis for vaccine production and for maintaining stock cultures.
Application: For The Detection & Isolation of Bordetella Pertussis & Bordetella Parapertussis . Also Used For The "Cough Plate" Method in Case of Whooping Cough
- Appearance: Cream to yellow homogeneous free flowing powder
- Gelling: Firm, comparable with 2.0% agar gel
- Color and Clarity of prepared medium: Basal Medium : Light yellow colored clear to slightly opalescent gel. After addition of glycerol and 15% v/v sterile defibrinated blood: Cherry red colored opaque gel forms in Petri plates
- Reaction of 4% w/v aqueous solution at 25°C. pH : 6.7±0.2
- Cultural characteristics observed with added Glycerol and 15% v/v sterile defibrinated blood and Bordetella Selective Supplement (FD004), after an incubation at 35-37°C for 3-4 days